Home
page up
about Bioserv Diagnostics
gastroenterology
reproductive immunology
risk pregnancy monitoring
list of assays
contact
accredited laboratory

LH ELISA from BIOSERV Diagnostics

FSH and LH are gonadotropins from the anterior lobe of the pituitary gland. Like the placentary hormone hCG and like TSH (Thyreoidea stimulating hormone) they are proteohormones of about 30 kD, all sharing the same α-subunit. The biological activity of these hormones is determined by the specific β-subunit.

The use of this FSH test for the determination of FSH and LH is indicated for the assessment of menstrual cycle disorders, fertility problems and the assessment of a hormonal substitution during the climacterium. Continuously high FSH and LH values are characteristic for a primary orvarial insufficiency.

With the BIOSERV hLH serum ELISA human Luteinizing hormone (hLH) is detected in human serum, for example for the exact determination of the ovulation time.

The LH ELISA from BIOSERV is a ready-to-use kit.

To order the test please refer to the BIOSERV catalogue number BS - 85 - 23.

For further information please send us an e-mail: info@bioserv-diagnostics.com

        EU registration number DE/CA80/IVD1685

Detailed information on the hLH ELISA from BIOSERV Diagnostics

The human Luteinizing Hormone (hLH) is synthetized in the anterior lobe of the pituitary gland. The synthesis and secretion is controlled by the hypothalamic Gonadotropin-Releasing-Hormone (Gn-RH). hLH is a glycoprotein with a molecular weight of about 30 kD. Like the other two gonadotropins hFSH (human Follicle Stimulating Hormone) and hCG (human Chorionic Gonadotropin) hLH consists of an α- and a β-subunit. The α-subunit is the same for all three gonadotropins and even for hTSH (human Thyreotropic Hormone), whereas the specific hormonal effects are due to the specific β-subunits.

In women hLH and hFSH are responsible for the modifications of the ovaries during the menstrual cycle. During the follicular phase the follicles mature under the influence of hFSH, hLH and Estrogens. During the first days the hFSH concentration in the blood rises slightly, reaching a maximum around the 11^th/12^th day. The hLH titer in the blood at first stays at a low level but begins then to raise rapidly with the hLH maximum, leading to a maximum around the 14^th/15^th day (middle of the menstrual cycle). Under the influence of this high hLH concentration the ovulation occurs. With the ovulation the luteal phase, during which hLH stimulates the formation of the Corpus luteum, begins. hLH also furthers the synthesis of progesterone and estrogens during the second half of the menstrual cycle. Increased hLH and hFSH values are a sign for a primary ovarial malfunction, normally linked to an amenorrhoe. In men hLH, in this context sometimes referred to as Interstitial Cell Stimulating Hormone (ICSH) together with hFSH positively influences the spermiogenesis as well as the synthesis of testerone by the Leydig’s cells in the testes. High hLH values may be causative of a testicular malfunction.

Fields of application

The LH serum ELISA from BIOSERV Diagnostics can be applied in the clinical practice for, among others:

• the diagnosis of primary and secondary amenorrhoea

• the determination of menstrual cycles without ovulation

• within the scope of the treatment of female infertility

• the diagnosis of the beginning climacterium

• the diagnosis of testicular malfunctions in men

• within the scope of the treatment of male infertility 

Sample material

Serum

Reference value range for the hLH concentration in serum

Reference: WHO 80/552

Adult women with ovulatory cycles:*         

                * comparative studies have shown a high correlation between the hLH-levels in serum and urine during the course of the ovulatory cycle  

For further information please send us an e-mail: info@bioserv-diagnostics.com

Back to the beginning of this page