Quantitative ELISA for the detection of anti-sperm antibodies (anti-spermatozoa antibodies, sperm antibodies). This anti-sperm ELISA has been optimized for the detection of anti-sperm antibodies in human seminal plasma.
Anti-sperm antibodies of the patient's sample material bind to sperm antigens immobilized on a 96-well-ELISA plate. In a second step enzyme-linked antibodies directed against human immunoglobulins are added. The antibody concentration in the patient's serum correlates directly with the extinction values of the subsequent photometric measurements.